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vectors expressed oct4  (Addgene inc)


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    Structured Review

    Addgene inc vectors expressed oct4
    Vectors Expressed Oct4, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vectors+expressed+oct4/pCE-hOCT3%2F4+(Plasmid+%2341813)/pm41660885-55-24-27
    Average 94 stars, based on 52 article reviews
    vectors expressed oct4 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    shRNA:

    Article Title: Generation of iPS cell line (ICGi040-A) from skin fibroblasts of a patient with ring small supernumerary marker chromosome 4.
    Article Snippet: 500 × 103 of the TAF16 fibroblasts were electroporated with 6 μg episomal vectors cocktail by Neon Transfection System (Thermo Fisher Scientific) at following conditions: 1650 V, 10 ms, 3 pulses in the volume 100 μl. .. The episomal vectors cocktail contained: reprogramming vectors expressed OCT4 (addgene #41813), MYC and LIN28 (addgene #41855), shRNA against p53 (addgene #41856), SOX2 and KLF4 (addgene #41814), EBNA1 (addgene # 41857). .. Cells were seeded on a mitomycin C-treated CD-1 mouse embryonic fibroblast in iPSC medium: DMEM/ F12,20% KSR, 1% GlutaMAX-I, 1% MEM NEAA, 1% Pen Strep, 0.1 mM 2-mercaptoethanol, and 10 ng/ml bFGF (all from Thermo Fisher Scientific).

    Article Title: A novel approach for simultaneous detection of structural and single-nucleotide variants based on a combination of chromosome conformation capture and exome sequencing
    Article Snippet: .. Reprogramming of human blood cells into iPS cells was performed by episomal vectors cocktail contained: reprogramming vectors expressed OCT4 (addgene #41813), MYC and LIN28 (addgene #41855), shRNA against p53 (addgene #41856), SOX2 and KLF4 (addgene #41814), EBNA1 (addgene # 41857), according to the previously described method . iPS cells were cultured as described before . ..

    Article Title: Combining chromosome conformation capture and exome sequencing for simultaneous detection of structural and single-nucleotide variants
    Article Snippet: .. Reprogramming of human peripheral blood mononuclear cells (PBMC) into iPS cells was performed by episomal vectors cocktail contained: reprogramming vectors expressed OCT4 (addgene #41,813), MYC and LIN28 (addgene #41,855), shRNA against p53 (addgene #41,856), SOX2 and KLF4 (addgene #41,814), EBNA1 (addgene # 41,857), according to the previously described method [ ]. .. Briefly, 5 × 10 5 PBMC were transfected with 1 μg of each vector using the Neon Transfection System (Thermo Fisher Scientific) under optimized conditions (1650 V, 10 ms, 3 pulses).

    Article Title: Combining chromosome conformation capture and exome sequencing for simultaneous detection of structural and single-nucleotide variants.
    Article Snippet: .. Exome enrichment was performed using KAPA HyperExome Probes and KAPA HyperCap kit according to the manufacturer’s instructions. iPS cells generation and differentiation Reprogramming of human peripheral blood mononuclear cells (PBMC) into iPS cells was performed by episomal vectors cocktail contained: reprogramming vectors expressed OCT4 (addgene #41,813), MYC and LIN28 (addgene #41,855), shRNA against p53 (addgene #41,856), SOX2 and KLF4 (addgene #41,814), EBNA1 (addgene # 41,857), according to the previously described method [14]. .. Briefly, 5 × 105 PBMC were transfected with 1 μg of each vector using the Neon Transfection System (Thermo Fisher Scientific) under optimized conditions (1650 V, 10 ms, 3 pulses).

    Cell Culture:

    Article Title: A novel approach for simultaneous detection of structural and single-nucleotide variants based on a combination of chromosome conformation capture and exome sequencing
    Article Snippet: .. Reprogramming of human blood cells into iPS cells was performed by episomal vectors cocktail contained: reprogramming vectors expressed OCT4 (addgene #41813), MYC and LIN28 (addgene #41855), shRNA against p53 (addgene #41856), SOX2 and KLF4 (addgene #41814), EBNA1 (addgene # 41857), according to the previously described method . iPS cells were cultured as described before . ..

    Dominant Negative Mutation:

    Article Title: Zooming into rearranged genome: applying pipeline of cytological, genomic, and transcriptomic methods for structural variant interpretation
    Article Snippet: Exome enrichment was carried out using the KAPA HyperExome Probes and KAPA HyperCap kit (Roche), following the manufacturer’s protocol. .. Reprogramming of patient blood cells into induced pluripotent stem (iPS) cells was performed by episomal vectors cocktail contained: reprogramming vectors expressed OCT4 (addgene #41813), MYC and LIN28 (addgene #41855), p53 carboxy-terminal dominant-negative fragment (addgene #41856), SOX2 and KLF4 (addgene #41814), EBNA1 (addgene # 41857), according to the previously described method . .. Briefly, 5 × 105 peripheral blood mononuclear cell were transfected with 1 μg of each vector using the Neon Transfection System (Thermo Fisher Scientific, USA) under optimized conditions (1650 V, 10 ms, 3 pulses).



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